Lactose Broth And Lactose Agar

Jual Lactose Broth
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Lactose Broth is frequently used as a pre-enrichment broth during testing of foods and dairy products for Salmonella. In processed foods, Salmonella species can be present in low concentrations and the cells may be found in a debilitated condition. Lactose Broth provides an environment favorable for the recovery of Salmonella. The media allows damaged cells to recover, dilutes toxic substances that may be present, and favors growth of Salmonella over other species.(2) Lactose Broth is included in many procedures for testing foods, dairy products, and other materials.

A pre-enrichment medium provides a higher ratio of Salmonella to non-Salmonella bacteria after incubation. Most non-Salmonella bacteria ferment lactose, while Salmonella does not. When lactose-fermenting bacteria metabolize lactose in the medium, the pH decreases, creating a bacteriostatic effect on competing microorganisms.

Lactose Broth is also used to detect coliforms in food, dairy products, and water.(1-4) The concentration of ingredients in Lactose Broth, when used to detect coliforms in samples, must remain the same after addition of the sample being tested. Lactose Broth with lecithin and Tween® formulations aid in neutralizing disinfectants or preservatives that may inhibit bacterial cells. Neutralization of these residues reduces their inhibitory effects and aids in the recovery of sub-lethally injured cells.